Dermatophyte Test Medium Base

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dermatophyte Test Medium Base

培养基配方
Composition per liter:
Agar............................................................................20.0g
Glucose........................................................................10.0g
Papaic digest of soybean meal..................................................10.0g
Cycloheximide...................................................................0.5g
Phenol Red......................................................................0.2g
Gentamycin sulfate..............................................................0.1g
Chlortetracycline...............................................................0.1g
pH 5.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except gentamycin sul-
fate and chlortetracycline, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add gentamycin sulfate and chlortetracycline. Mix
thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation and cultivation of pathogenic fungi
from cutaneous sources.