Derxia gummosa Medium

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Derxia gummosa Medium

培养基配方
Composition per liter:
Agar ...............................................................................20.0g
Starch .............................................................................20.0g
MgSO4 ·7H2O.........................................................................0.2g
KH2PO4 .............................................................................0.15g
NaHCO3 ..............................................................................0.1g
K2HPO4 .............................................................................0.05g
CaCl2 ..............................................................................0.02g
Na2MoO4 ·2H2O......................................................................2.0mg
Bromthymol Blue solution ...........................................................5.0mL
FeCl3 ·6H2O (10% solution).........................................................0.1mL
pH 6.9 ± 0.2 at 25°C
Bromthymol Blue Solution:
Composition per 10.0mL:
Bromthymol Blue.....................................................................0.5g
Ethanol...........................................................................10.0mL
Preparation of Bromthymol Blue Solution: Add Bromthymol
Blue to 10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Derxia gummosa.