Dermatophyte Test Medium

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dermatophyte Test Medium

培养基配方
Composition per liter:
Agar..........................................................................20.0g
Enzymatic digest of soybean meal .............................................10.0g
Glucose.......................................................................10.0g
Cycloheximide..................................................................0.5g
Phenol Red.....................................................................0.2g
Selective supplement solution ...............................................10.0mL
pH 5.5 ± 0.2 at 25°C
Source:  This medium is available from Acumedia, Neogen Corp.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Selective Supplement Solution:
Composition per 10.0mL:
Gentamicin ..................................................................0.1g
Chlortetracycline ...........................................................0.1g
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Distribute into tubes or flasks. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically add 10.0mL selective supplement solution.
Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use:  For the selective isolation of dermatophytic fungi.