Dermasel Agar Base

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dermasel Agar Base

培养基配方
Composition per liter:
Glucose ....................................................................20.0g
Agar .......................................................................14.5g
Papaic digest of soybean meal...............................................10.0g
Antibiotic inhibitor.......................................................10.0mL
pH 6.8–7.0 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Antibiotic Inhibitor:
Composition per 10.0mL:
Cycloheximide...............................................................0.4g
Chloramphenicol............................................................0.05g
Acetone...................................................................10.0mL
Preparation of Antibiotic Inhibitor: Add cycloheximide and
chloramphenicol to 10.0mL of acetone. Mix thoroughly.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 990.0mL. Mix thoroughly. Gently heat and
bring to boiling. Do not overheat. Add antibiotic inhibitor. Mix thor-
oughly. Autoclave for 10 min at 15 psi pressure–121°C. Pour into ster-
ile Petri dishes.
Use: For the isolation and cultivation of dermatophytic fungi isolated
from hair, nails, or skin scrapings.