Decarboxylase Medium Base, Falkow

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Decarboxylase Medium Base, Falkow

培养基配方
Composition per liter:
Amino acid (arginine, lysine, or ornithine) ......................................5.0g
Peptone ..........................................................................5.0g
Yeast extract.....................................................................3.0g
Glucose...........................................................................1.0g
Bromcresol Purple................................................................0.02g
Mineral oil ...................................................................200.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except mineral oil, to
distilled/deionized water and bring volume to 1.0L. For amino acid,
use  L- arginine,  L- lysine, or  L- ornithine. Mix thoroughly. Distribute into
screw-capped tubes in 5.0mL volumes. Autoclave medium and miner-
al oil separately for 15 min at 15 psi pressure–121°C. After inoculation,
overlay medium with 1.0mL of sterile mineral oil per tube.
Use: For the cultivation and differentiation of bacteria based on their
ability to decarboxylate a specific amino acid. Bacteria that decarbox-
ylate arginine, lysine, or ornithine turn the medium turbid purple.