Decarboxylase HiVeg Broth Base, Moeller

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Decarboxylase HiVeg Broth Base, Moeller

培养基配方
Composition per liter:
Plant extract.................................................................5.0g
Plant peptone ................................................................5.0g
Glucose.......................................................................0.5g
Bromcresol Purple............................................................0.01g
Cresol Red ..................................................................5.0mg
Pyridoxal....................................................................5.0mg
Amino acid solution........................................................100.0mL
pH 6.0 ± 0.2 at 25°C
Source: This medium wihout amino acid is available as a premixed
powder from HiMedia.
Amino Acid Solution:
Composition per 100.0mL:
L- arginine,  L- lysine, or  L- ornithine....................................10.0g
Preparation of Amino Acid Solution: Add amino acid to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components and bring volume to
900.0L. For amino acid, use  L- arginine,  L- lysine, or  L- ornithine and add
100.0ml of a 10% solution. Mix thoroughly. Distribute into screw-
capped tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: As a basal medium for the cultivation and differentiation of bac-
teria based on their ability to decarboxylate amino acids. The medium
is supplemented with specific  L -amino acids for testing decarboxylase
activity on that amino acid. Bacteria that decarboxylate arginine,
lysine, or ornithine turn the medium turbid purple.