Decarboxylase Medium, Ornithine Modified

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Decarboxylase Medium, Ornithine Modified

培养基配方
Composition per liter:
L- Ornithine...............................................................10.0g
Meat peptone................................................................5.0g
Yeast extract...............................................................3.0g
Bromcresol Purple solution ................................................5.0mL
pH 5.5 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple .........................................................0.2g
Ethanol..................................................................50.0mL
Preparation of Bromcresol Purple Solution:  Add Bromcresol
Purple to ethanol. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until dis-
solved. Adjust pH to 5.5 with HCl or NaOH. Distribute into screw-
capped tubes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of bacteria based on their
ability to decarboxylate ornithine. Bacteria that decarboxylate orni-
thine turn the medium turbid purple.