Decarboxylase HiVeg Agar Base

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Decarboxylase HiVeg Agar Base

培养基配方
Composition per liter:
Agar ..........................................................................15.0g
Plant peptone...................................................................5.0g
Yeast extract...................................................................3.0g
Glucose ........................................................................1.0g
Bromcresol Purple ..............................................................0.02
Amino acid solution..........................................................100.0mL
pH 6.5 ± 0.2 at 25°C
Source: This medium wihout amino acid is available as a premixed
powder from HiMedia.
Amino Acid Solution:
Composition per 100.0mL:
L- arginine,  L- lysine, or  L- ornithine..................................10.0g
Preparation of Amino Acid Solution: Add amino acid to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components and bring volume to
900.0L. For amino acid, use  L- arginine,  L- lysine, or  L- ornithine and add
100.0ml of a 10% solution. Mix thoroughly. Distribute into screw-
capped tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: As a basal medium for the cultivation and differentiation of bac-
teria based on their ability to decarboxylate amino acids. The medium
is supplemented with specific  L -amino acids for testing decarboxylase
activity on that amino acid. Amino acids are added to a final concen-
tration of 0.5 percent.