Davis and Mingioli Medium, Modified

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Davis and Mingioli Medium, Modified

培养基配方
Composition per liter:
K2HPO4 ..........................................................................7.0g
KH2PO4 ..........................................................................2.0g
(NH4)2SO4 .......................................................................1.0g
Na citrate·2H2O ................................................................0.5g
MgSO4 ·7H2O.....................................................................0.1g
Glucose solution...............................................................10.0mL
Streptomycin solution..........................................................10.0mL
Additives solution.............................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose ........................................................................4.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Streptomycin Solution:
Composition per 10.0mL:
Streptomycin.....................................................................4.0g
Preparation of Streptomycin Solution:  Add streptomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix well. Filter
sterilize.
Additives Solution:
Composition per 10.0mL:
DL- Threonine.....................................................................0.1g
DL- or  L- Leucine................................................................0.1g
Thiamine·HCl ...................................................................0.5mg
Preparation of Additives Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except glucose solu-
tion, streptomycin solution, and additives solution—to distilled/deion-
ized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to
7.2. Gently heat and bring to boiling. Autoclave for 25 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add sterile glucose solution,
streptomycin solution, and additives solution. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Escherichia coli.