Davis and Mingioli Medium, Modified

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Davis and Mingioli Medium, Modified

培养基配方
Composition per liter:
Agar .........................................................................15.0g
K2HPO4 ........................................................................7.0g
Lactose........................................................................2.0g
(NH4)2SO4 .....................................................................1.0g
KH2PO4 .......................................................................0.91g
MgSO4 ·7H2O...................................................................0.1g
Tap water....................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Escherichia coli.