Davis and Mingioli Medium A

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Davis and Mingioli Medium A
Composition per liter:
K2HPO4 ..........................................................................7.0g
KH2PO4 ..........................................................................3.0g
(NH4)2SO4 .......................................................................1.0g
Sodium citrate·3H2O.............................................................0.5g
MgSO4 ·7H2O.....................................................................0.1g
Glucose solution...............................................................10.0mL
Amino acid solution............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose..........................................................................2.5g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Amino Acid Solution:
Composition per 10.0mL:
L -Leucine....................................................................40.0mg
L -Histidine .................................................................20.0mg
L -Methionine.................................................................20.0mg
Preparation of Amino Acid Solution:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except glucose solu-
tion and amino acid solution, to distilled/deionized water and bring
volume to 980.0mL. Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
10.0mL of sterile glucose solution and 10.0mL of sterile amino acid so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Escherichia coli.