Davis and Mingioli Glucose Minimal Medium

[所属分类:培养基配方] [发布时间:2020-6-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Davis and Mingioli Glucose Minimal Medium

培养基配方
Composition per liter:
Agar ..........................................................................15.0g
K2HPO4 .........................................................................7.0g
KH2PO4 .........................................................................3.0g
(NH4)2SO4 ......................................................................1.0g
Sodium citrate·3H2O............................................................0.5g
MgSO4 ·7H2O....................................................................0.1g
L- Arginine ...................................................................0.02g
L- Tryptophan..................................................................0.02g
Glucose solution .............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose.........................................................................2.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium:  Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add sterile glucose solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation and maintenance of Escherichia coli.