Cytophaga fermentans Medium

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cytophaga fermentans Medium

培养基配方
Composition per liter:
NaCl..........................................................................30.0g
Agar...........................................................................5.0g
NaHCO3 ........................................................................5.0g
KH2PO4 ........................................................................1.0g
NH4Cl .........................................................................1.0g
MgCl2 ·6H2O ..................................................................0.5g
Yeast extract..................................................................0.3g
Na2S·9H2O.....................................................................0.1g
CaCl2 ........................................................................0.04g
Ferric citrate (4mM solution).................................................5.0mL
Trace elements solution.......................................................2.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 100.0mL:
H3BO3 .......................................................................0.28g
MnSO4 ·6H2O.................................................................0.21g
Na2MoO4 ·2H2O..............................................................0.075g
Zn(NO3)2 ·6H2O ............................................................0.025g
CoCl2 ·6H2O.................................................................0.02g
Cu(NO3)2 ·3H2O..............................................................0.02g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the cultivation of agar-digesting Cytophaga fermentans.