Cytophaga hutchinsonii Agar

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cytophaga hutchinsonii Agar

培养基配方
Composition per liter:
Agar.........................................................................15.0g
Pancreatic digest of casein...................................................3.0g
CaCl2 ·2H2O ................................................................1.36g
Yeast extract.................................................................1.0g
Cellobiose solution.........................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Cellobiose Solution:
Composition per 50.0mL:
D- Cellobiose...............................................................6.0g
Preparation of Cellobiose Solution: Add cellobiose to distilled/
deionized water and bring volume to 50.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except cellobiose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of
sterile cellobiose solution. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the cultivation and maintenance of Cytophaga aurantiaca
and Cytophaga hutchinsonii.