Cytophaga agarovorans Broth (LMG Medium 99)

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Bio-Engineering Co.,Ltd

Cytophaga agarovorans Broth
(LMG Medium 99)

培养基配方
Composition per 1001.0mL:
NaCl.............................................................................30.0g
KH2PO4 ...........................................................................1.0g
MgSO4 ·7H2O......................................................................1.0g
NH4Cl ............................................................................1.0g
Yeast extract.....................................................................1.0g
Agar .............................................................................1.0g
CaCl2 ·2H2O....................................................................50.0mg
FeCl3 ·H2O.....................................................................1.25mg
Glucose solution ...............................................................10.0mL
NaHCO3  solution ...............................................................10.0mL
Na2S·9H2O solution..............................................................1.0mL
Glucose Solution:
Composition per 10.0mL:
D- Glucose.......................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ...........................................................................5.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O........................................................................1.0g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, NaHCO3  solution, and Na2S·9H2O solution, to distilled/deionized
water and bring volume to 980.0mL. Add 1.0g of agar as a detoxifying
agent. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically add 10.0mL of sterile glucose solution, 10.0mL of sterile
NaHCO3  solution, and 1.0mL of sterile Na2S·9H2O solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Cytophaga agarovorans.