Cytophaga agarovorans Agar (LMG Medium 99)

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cytophaga agarovorans Agar
(LMG Medium 99)

培养基配方
Composition per 1001.0mL:
NaCl.............................................................................30.0g
Agar.............................................................................15.0g
KH2PO4 ...........................................................................1.0g
MgSO4 ·7H2O......................................................................1.0g
NH4Cl ............................................................................1.0g
Yeast extract.....................................................................1.0g
CaCl2 ·2H2O ...................................................................50.0mg
FeCl3 ·H2O.....................................................................1.25mg
Glucose solution................................................................10.0mL
NaHCO3  solution................................................................10.0mL
Na2S·9H2O solution..............................................................1.0mL
Glucose Solution:
Composition per 10.0mL:
D- Glucose........................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ...........................................................................5.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O.........................................................................1.0g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, NaHCO3  solution, and Na2S·9H2O solution, to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 10.0mL of sterile glucose solution,
10.0mL of sterile NaHCO3  solution, and 1.0mL of sterile Na2S·9H2O
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Cytophaga agarovorans.