Cytophaga Agarase Broth

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cytophaga Agarase Broth

培养基配方

Composition per liter:
Agar.......................................................................1.0g
KH2PO4 ....................................................................1.0g
MgSO4 ·7H2O...............................................................0.5g
NH4Cl .....................................................................0.5g
CaCl2 ·H2O...............................................................0.02g
Vishniac and Santer trace element mixture ................................0.2mL
pH 7.2 ± 0.2 at 25°C
Vishniac and Santer Trace Element Mixture:
Composition per liter:
Ethylenediamine tetraacetic acid (EDTA)....................................50.0g
ZnSO4 ·7H2O...............................................................22.0g
CaCl2 .....................................................................5.54g
MnCl2 ·4H2O ..............................................................5.06g
FeSO4 ·7H2O...............................................................4.99g
CoCl2 ·6H2O...............................................................1.61g
CuSO4 ·5H2O...............................................................1.57g
(NH4)6Mo7O24 ·4H2O.........................................................1.1g
Preparation of Vishniac and Santer Trace Element Mix-
ture: Add components to distilled/deionized water and bring volume
to 1.0L. Adjust pH to 6.0 with KOH. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Adjust pH to 7.2. Mix thoroughly. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation of Cytophaga flevensis.