Cystine HiVeg Agar Base with Hemoglobin

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cystine HiVeg Agar Base with Hemoglobin

培养基配方
Composition per liter:
Agar........................................................................15.0g
Plant infusion..............................................................10.0g
Plant peptone No.3..........................................................10.0g
Glucose ....................................................................10.0g
NaCl.........................................................................5.0g
L -Cystine...................................................................1.0g
Hemoglobin solution.......................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium, wihout hemoglobin, is available as a premixed
powder from HiMedia.
Hemoglobin Solution:
Composition per 100.0mL:
Bovine hemoglobin...........................................................2.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to
distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except hemoglobin so-
lution, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Add 100.0mL sterile hemoglobin
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Gram-negative cocci and other fastidious
pathogens. For the cultivation of Francicella tularensis.