Cystine Tellurite Blood Agar

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cystine Tellurite Blood Agar

培养基配方
Composition per liter:
Heart infusion agar............................................................900.0mL
K2TeO3 solution.................................................................75.0mL
Rabbit blood....................................................................25.0mL
L- Cystine .....................................................................22.0mg
pH 7.4 ± 0.2 at 25°C
Heart Infusion Agar:
Composition per 900.0mL:
Beef heart, solids from infusion................................................500.0g
Agar ............................................................................20.0g
Tryptose ........................................................................10.0g
Yeast extract.....................................................................5.0g
NaCl..............................................................................5.0g
Preparation of Heart Infusion Agar:  Add components to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
K2TeO3 Solution:
Composition per 100.0mL:
K2TeO3 ...........................................................................0.3g
Preparation of K2TeO3 Solution: Add K2TeO3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add sterile K2TeO3 solution, sterile rabbit
blood, and sterile, solid  L- cystine to sterile, cooled heart infusion agar. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation, differentiation, and cultivation of Corynebacte-
rium diphtheriae. Corynebacterium diphtheriae appears as dark gray to
black colonies.