Cystine Heart Agar with Rabbit Blood

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cystine Heart Agar with Rabbit Blood

培养基配方
Composition per liter:
Beef heart, solids from infusion..........................................500.0g
Agar.......................................................................15.0g
Glucose....................................................................10.0g
Proteose peptone...........................................................10.0g
NaCl........................................................................5.0g
L- Cystine..................................................................1.0g
Rabbit blood, defibrinated................................................50.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components, except rabbit blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–60°C. Aseptically add 50.0mL of sterile, defibri-
nated rabbit blood. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the cultivation and maintenance of Francisella tularensis and
Francisella philomiragia. Without the hemoglobin enrichment, it sup-
ports excellent growth of Gram-negative cocci and other pathogenic
microorganisms.