Cystine Heart Agar

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cystine Heart Agar

培养基配方
Composition per liter:
Beef heart, solids from infusion...............................................500.0g
Agar............................................................................15.0g
Glucose.........................................................................10.0g
Proteose peptone................................................................10.0g
NaCl.............................................................................5.0g
L- Cystine.......................................................................1.0g
Hemoglobin solution ..........................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Hemoglobin Solution:
Composition per 100.0mL:
Hemoglobin......................................................................2.0g
Preparation of Hemoglobin Solution: Add hemoglobin to cold
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly by shaking for 10–15 min. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–60°C.
Preparation of Medium:  Add components, except hemoglobin so-
lution, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50–60°C. Aseptically add 100.0mL of sterile
cooled hemoglobin solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Francisella tularensis and
Francisella philomiragia. Without the hemoglobin enrichment, it sup-
ports excellent growth of Gram-negative cocci and other pathogenic
microorganisms.