CYS Medium (DSMZ Medium 1108)

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CYS Medium
(DSMZ Medium 1108)

培养基配方
Composition per liter:
Gelrite ..................................................................8.0g
NZ Case (Wako pure chemicals, Japan) .....................................3.0g
NaCl .....................................................................3.0g
Yeast extract ............................................................2.0g
Soluble starch ...........................................................1.0g
MgCl2 ..................................................................0.125g
CaCl2 ..................................................................0.025g
FeSO4 ·7H2O.............................................................0.01g
Solution A...............................................................0.1mL
Solution B...............................................................0.1mL
Solution C...............................................................0.1mL
Solution D...............................................................0.1mL
Solution E ..............................................................0.1mL
Solution F...............................................................0.1mL
Solution G...............................................................0.1mL
pH 7.5 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
Na2MoO4 ·4H2O ..........................................................1.2g
Preparation of Solution A:  Add Na2MoO4 ·4H2O to 100.0mL of
distilled/deionized water. Mix thoroughly.
Solution B:
Composition per 100.0mL:
VOSO4 ·2H2O.........................................................0.1mg
Preparation of Solution B:  Add VOSO4 ·2H2O to 100.0mL of dis-
tilled/deionized water. Mix thoroughly.
Solution C:
Composition per 100.0mL:
MnCl2 ·4H2O.........................................................0.5g
Preparation of Solution C:  Add MnCl2 ·4H2O to 100.0mL of dis-
tilled/deionized water. Mix thoroughly.
Solution D:
Composition per 100.0mL:
ZnSO4 ·7H2O........................................................0.06g
Preparation of Solution D:  Add ZnSO4 ·7H2O to 100.0mL of dis-
tilled/deionized water. Mix thoroughly.
Solution E:
Composition per 100.0mL:
CuSO4 ·5H2O.......................................................0.015g
Preparation of Solution E:  Add CuSO4 ·5H2O to 100.0mL of dis-
tilled/deionized water. Mix thoroughly.
Solution F:
Composition per 100.0mL:
CoCl2 ·6H2O ......................................................0.8g
Preparation of Solution F:  Add CoCl2 ·6H2O to 100.0mL of dis-
tilled/deionized water. Mix thoroughly.
Solution G:
Composition per 100.0mL:
NiCl2 ·6H2O..........................................................0.02g
Preparation of Solution G:  Add NiCl2 ·6H2O to 100.0mL of dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components, except solutions A–G,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Adjust pH to 7.5. Individually and in order add solutions A–G.
After the addition of each solution mix thoroughly. Bring final volume
to 1.0L with distilled/deionized water. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the cultivation of Saccharomyces cerevisiae.