Cycloserine Cefoxitin Egg Yolk Fructose Agar

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cycloserine Cefoxitin Egg
Yolk Fructose Agar

培养基配方
Composition per liter:
Proteose peptone No.2.......................................................40.0g
Agar .......................................................................25.0g
Fructose.....................................................................6.0g
Na2HPO4 .....................................................................5.0g
NaCl.........................................................................2.0g
KH2PO4 ......................................................................1.0g
MgSO4 ·7H2O.................................................................0.1g
Egg yolk emulsion.........................................................100.0mL
Antibiotic solution........................................................10.0mL
Neutral Red solution........................................................3.0mL
Hemin solution..............................................................1.0mL
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................11
Whole chicken egg ............................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs. Sep-
arate yolks from whites for 11 eggs. Mix egg yolks with 1 chicken egg.
Antibiotic Solution:
Composition per 10.0mL:
Cycloserine....................................................................0.5g
Cefoxitin....................................................................0.016g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Neutral Red Solution:
Composition per 10.0mL:
Neutral Red...................................................................0.1g
Ethanol.....................................................................10.0mL
Preparation of Neutral Red Solution: Add Neutral Red to
10.0mL of ethanol. Mix thoroughly.
Hemin Solution:
Composition per 100.0mL:
Hemin .........................................................................0.5g
NaOH (1N solution)...........................................................10.0mL
Preparation of Hemin Solution:  Add hemin to 10.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium: Add components, except egg yolk emul-
sion and antibiotic solution, to distilled/deionized water and bring vol-
ume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile egg yolk emulsion and antibiotic solution. Mix
thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation and cultivation of Clostridium difficile
from feces.