CYE-ACES Agar (DSMZ Medium 585)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CYE-ACES Agar
(DSMZ Medium 585)

培养基配方
Composition per liter:
Solution A...................................................................490.0mL
Solution B...................................................................490.0mL
Soluiton C....................................................................10.0mL
Solution D....................................................................10.0mL
pH 6.9 ± 0.1 at 25°C
Solution A:
Composition  per 490mL:
Yeast extract..................................................................10.0g
ACES (N-2-acetamido-2-aminoethane-
sulfonic acid).................................................................10.0g
Activated charcoal .............................................................2.0g
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 490.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°C.
Solution B:
Composition  per 490mL:
Agar ...........................................................................15.0g
Preparation of Solution B : Add agar to distilled/deionized water
and bring volume to 490.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C.
Solution C:
Composition per 10.0mL:
L -Cysteine·HCl·H2O ...........................................................0.4g
Preparation of Solution C:  Add  L -cysteine·HCl·H2O to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Solution D:
Composition per 10.0mL:
Fe4(PO4)2 .....................................................................0.25g
Preparation of Solution D:  Add Fe4(PO4)2 to distilled/deionized
water and bring volume to 10.0mL. Heat to 50–55°C to dissolve
Fe4(PO4)2 . Mix thoroughly. Filter sterilize. Store in the dark. Do not
use if chemical loses its green color and becomes brown or yellow.
Preparation of Medium: Add 10.0mL solution C and then 10.0mL
solution D to 490.0mL solution A. Adjust the pH 6.9 ± 0.05 at 50°C by
adding 4.0 to 4.5 mL of sterile 1.0N KOH. The pH of the medium is
critical. Finally, add 490.0mL solution B. Mix thoroughly. Swirl medi-
um in flask during dispensing to Petri dishes or tubes to keep charcoal
suspended. Pour into Petri dishes or aseptically distribute into sterile
tubes.
Use: For the cultivation of Afipia clevelandensis, Afipia broomeae,
Afipia felis, Legionella pneumophila, Legionella longbeachae, and
Xylella fastidiosa.