Cyclohexanone Medium

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cyclohexanone Medium

培养基配方
Composition per liter:
NH4NO3 .........................................................................3.0g
K2HPO4 ........................................................................0.25g
MgSO4 ·7H2O....................................................................0.2g
CaCl2 ·2H2O...................................................................0.01g
FeCl3 ·6H2O...................................................................1.0mg
Cyclohexanone..................................................................1.0mL
Preparation of Medium: Add components, except cyclohexanone,
to distilled/deionized water and bring volume to 999.0mL. Mix thor-
oughly. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi
pressure–121°C. Filter sterilize cyclohexanone. Aseptically add 1.0mL
of cyclohexanone. Mix thoroughly.
Use:  For the cultivation and maintenance of Nocardia species and
other bacteria that can utilize cyclohexanone as a carbon source.