Cyclohexanecarboxylic Acid Medium

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cyclohexanecarboxylic Acid Medium

培养基配方
Composition per liter:
K2HPO4 ........................................................................3.5g
Cyclohexanecarboxylic acid.....................................................2.0g
KH2PO4 ........................................................................1.5g
NH4NO3 ........................................................................1.0g
MgSO4 ·7H2O...................................................................0.5g
Yeast extract..................................................................0.1g
CaCl2 ·2H2O..................................................................0.01g
FeCl3 ·6H2O..................................................................0.01g
NaMoO4 ·7H2O.................................................................0.01g
ZnSO4 ·7H2O..................................................................0.01g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Alcaligenes faecalis and
other bacteria that can utilize cyclohexanecarboxylic acid as a carbon
source.