Cyclohexanecarboxylic Acid Broth

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cyclohexanecarboxylic Acid Broth

培养基配方
Composition per liter:
Cyclohexanecarboxylic acid.................................................5.0g
(NH4)2SO4 .................................................................1.0g
KH2PO4 ....................................................................1.0g
K2HPO4 ....................................................................1.0g
MgSO4 ·7H2O................................................................0.2g
Yeast extract...............................................................0.1g
FeSO4 ·7H2O..............................................................10.0mg
CaCl2 ·2H2O ..............................................................2.0mg
MnSO4 ·4H2O...............................................................2.0mg
ZnSO4 ·7H2O...............................................................2.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation of Corynebacterium cyclohexanicum and Sac-
charomyces cerevisiae.