Cyclohexanecarboxylic Acid Agar

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cyclohexanecarboxylic Acid Agar

培养基配方
Composition per liter:
Agar, noble....................................................................15.0g
Cyclohexanecarboxylic acid......................................................5.0g
(NH4)2SO4 ......................................................................1.0g
KH2PO4 .........................................................................1.0g
K2HPO4 .........................................................................1.0g
MgSO4 ·7H2O....................................................................0.2g
Yeast extract...................................................................0.1g
FeSO4 ·7H2O..................................................................10.0mg
CaCl2 ·2H2O ..................................................................2.0mg
MnSO4 ·4H2O...................................................................2.0mg
ZnSO4 ·7H2O...................................................................2.0mg
pH7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Corynebacterium cyclo-
hexanicum and Saccharomyces cerevisiae.