CYC Agar

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CYC Agar

培养基配方
Composition per liter:
Sucrose......................................................................30.0g
Agar ........................................................................16.0g
Vitamin assay casamino acids..................................................6.0g
NaNO3 ........................................................................2.0g
Yeast extract.................................................................2.0g
Magnesium glycerophosphate ...................................................0.5g
KCl...........................................................................0.5g
K2SO4 .......................................................................0.35g
FeSO4 .......................................................................0.01g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Pseudonocardia thermo-
phila, Saccharomonospora caesia, Saccharomonospora viridis, Sac-
charopolyspora hirsuta, Saccharopolyspora rectivirgula, Streptomy-
ces  thermogriseoviolaceus,  Streptomyces  thermohygroscopicus,
Streptomyces thermovulgaris, Thermoactinomyces candidus, Thermo-
actinomyces putidus, Thermoactinomyces sacchari, Thermoactinomy-
ces thalpophilus, Thermoactinomyces vulgaris, and Thermomono-
spora fusca.