CYA Agar with Arginine and p-Aminobenzoic Acid (ATCC Medium 2033)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CYA Agar with Arginine and p-Aminobenzoic Acid
(ATCC Medium 2033)

培养基配方
Composition per liter:
Agar...........................................................................15.0g
Yeast extract...................................................................5.0g
NaNO3 ..........................................................................3.0g
K2HPO4 .........................................................................1.0g
KCl.............................................................................0.5g
MgSO4 ·7H2O....................................................................0.5g
Arginine........................................................................0.2g
FeSO4 ·7H2O...................................................................0.01g
p-Aminobenzoic acid.............................................................1mg
Sucrose solution..............................................................100.0mL
pH 7.3 ± 0.2 at 25°C
Sucrose Solution:
Composition per 100.0mL:
Sucrose.........................................................................30.0g
Preparation of Sucrose Solution: Add sucrose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Add components, except sucrose solution,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add sterile sucrose solution. Mix thoroughly. Pour into sterile
Petri dishes or leave in tubes.
Use:  For the isolation and cultivation of heat-resistant filamentous
fungi (molds) from foods.