CYC Medium

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CYC Medium

培养基配方
Composition per liter:
Sucrose......................................................................30.0g
Casamino acids, vitamin free..................................................6.0g
NaNO3 ........................................................................3.0g
Yeast extract.................................................................2.0g
K2HPO4 .......................................................................1.0g
MgSO4 ·7H2O..................................................................0.5g
KCl...........................................................................0.5g
FeSO4 ·7H2O.................................................................0.01g
Antibiotic solution ........................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide................................................................0.05g
Novobiocin..................................................................0.025g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile anti-
biotic solution. Mix thoroughly. Aseptically distribute into sterile
tubes.
Use: For the isolation and cultivation of Thermoactinomyces species.