CTLM Medium

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CTLM Medium

培养基配方
Composition per 1100.0mL:
Beef liver, infusion from.......................................................125.0g
Tryptose.........................................................................25.0g
Proteose peptone..................................................................2.5g
L -Cysteine·HCl ................................................................1.75g
Maltose .........................................................................1.25g
NaCl.............................................................................1.25g
Agar.............................................................................1.15g
L -Ascorbic acid.................................................................0.25g
NaHCO3 .........................................................................0.075g
Horse serum, heat inactivated..................................................100.0mL
Ringer's salt solution, 10× ...................................................75.0mL
pH 7.3 ± 0.2 at 25°C
Ringer's Salt Solution, 10×:
Composition per 100.0mL:
NaCl..............................................................................9.0g
KCl..............................................................................0.42g
CaCl2 ...........................................................................0.24g
Preparation of Ringer's Salt Solution, 10× : Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.3. Gently heat and bring to boiling. Autoclave for 25
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL
of sterile, heat-inactivated horse serum. Mix thoroughly. Aseptically
distribute into sterile, screw-capped tubes or flasks.
Use: For the cultivation of Trichomonas gallinae.