​CTLM Medium

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CTLM Medium

培养基配方
Composition per 1100.0mL:
Beef liver, infusion from..............................................125.0g
Tryptose ...............................................................25.0g
Proteose peptone.........................................................2.5g
L -Cysteine·HCl........................................................1.75g
Maltose.................................................................1.25g
NaCl....................................................................1.25g
Agar ...................................................................1.15g
L -Ascorbic acid........................................................0.25g
NaHCO3 ................................................................0.075g
Horse serum, heat inactivated.........................................100.0mL
Ringer's salt solution, 10×...........................................75.0mL
pH 7.0 ± 0.2 at 25°C
Ringer's Salt Solution, 10×:
Composition per 100.0mL:
NaCl....................................................................9.0g
KCl....................................................................0.42g
CaCl2 .................................................................0.24g
Preparation of Ringer's Salt Solution, 10× : Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-ly.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.0. Gently heat and bring to boiling. Autoclave for 25
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL
of sterile, heat-inactivated horse serum. Mix thoroughly. Aseptically
distribute into sterile, screw-capped tubes or flasks.
Use: For the cultivation of Monocercomonas colubrorum, Tet-
ratrichomonas gallinarum, Tritrichomonas foetus, and T. mobilensis.