CTT Medium

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CTT Medium

培养基配方
Composition per liter:
Agar...............................................................................15.0g
Pancreatic digest of casein........................................................10.0g
Tris(hydroxymethyl)aminomethane buffer.............................................1.21g
Potassium phosphate buffer (1 mM, pH 7.6)...........................................1.0L
Magnesium sulfate solution........................................................10.0mL
pH 7.6 ± 0.2 at 25°C
Magnesium Sulfate Solution:
Composition per 10.0mL:
MgSO4 ·7H2O........................................................................2.0g
Preparation of Magnesium Sulfate Solution: Add MgSO4 ·7H2O
to 10.0mL of distilled/deionized water. Mix thoroughly.
Preparation of Medium:  Combine components. Mix thoroughly.
Adjust pH to 7.6. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or leave in tubes.
Use: For the cultivation of myxobacteria.