CT Agar

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CT Agar

培养基配方
Composition per liter:
Agar ........................................................................20.0g
Pancreatic digest of casein..................................................20.0g
MgSO4 ·7H2O..................................................................2.0g
Potassium phosphate buffer (0.02M solution, pH 7.6) ..........500.0mL
pH 7.6 ± 0.2 at 25°C
Preparation of Medium:  Add agar, pancreatic digest of casein, and
MgSO4 ·7H2O to distilled/deionized water and bring volume to
500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
agar–pancreatic digest of casein-MgSO4 ·7H2O solution and potassium
phosphate buffer solution separately for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically combine the two solutions. Asepti-
cally add sterile components. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of myxobacteria.