CT Broth

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CT Broth

培养基配方
Composition per liter:
Pancreatic digest of casein...............................................20.0g
MgSO4 ·7H2O...............................................................2.0g
Potassium phosphate
buffer (0.02M solution, pH 7.6).........................................500.0mL
pH 7.6 ± 0.2 at 25°C.....
Preparation of Medium:  Add pancreatic digest of casein and
MgSO4 ·7H2O to distilled/deionized water and bring volume to
500.0mL. Mix thoroughly. Autoclave pancreatic digest of casein-
MgSO4 ·7H2O solution and potassium phosphate buffer solution sepa-
rately for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically
combine the two solutions. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of myxobacteria.