CT Agar (Caprylate Thallous Agar)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CT Agar
(Caprylate Thallous Agar)

培养基配方
Composition per liter:
Solution A................................................................500.0mL
Solution B................................................................500.0mL
pH 7.2 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
K2HPO4 .....................................................................2.61g
KH2PO4 .....................................................................0.68g
Thallous sulfate............................................................0.25g
MgSO4 ·7H2O................................................................0.12g
CaCl2 ·2H2O ..............................................................0.016g
Trace elements solution....................................................10.0mL
Yeast extract...............................................................2.0mL
Caprylic acid...............................................................1.1mL
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 7.2
with NaOH. Autoclave for 20 min at 10 psi pressure–115°C.
Trace Elements Solution:
Composition per liter:
H3PO4 .......................................................................1.96g
FeSO4 ·7H2O................................................................0.056g
ZnSO4 ·4H2O................................................................0.029g
CuSO4 ·5H2O................................................................0.025g
MnSO4 ·4H2O................................................................0.022g
H3BO3 .......................................................................6.2mg
Co(NO3)2 ·6H2O..............................................................3.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Store at 4°C.
Solution B:
Composition per liter:
Agar.......................................................................15.0g
NaCl........................................................................7.0g
(NH4)2SO4 ..................................................................1.0g
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Adjust pH to 7.2. Autoclave for 20 min at 10 psi pres-
sure–115°C.
Preparation of Medium: Aseptically combine 500.0mL of sterile
solution A and 500.0mL of sterile solution B. Mix thoroughly. Pour
into sterile Petri dishes in 25.0–30.0mL volumes.
Use: For the isolation and cultivation of the Serratia species.