CS Vitamin B 12 Agar

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CS Vitamin B 12  Agar

培养基配方
Composition per liter:
Glucose .....................................................................20.0g
K2SO4 .......................................................................20.0g
Agar ........................................................................15.0g
Sodium acetate...............................................................12.0g
Vitamin assay casamino acids.................................................10.0g
Papaic digest of soybean meal.................................................5.0g
Sodium thioglycolate..........................................................1.7g
K2HPO4 .......................................................................1.0g
KH2PO4 .......................................................................1.0g
Ribonucleic acid .............................................................1.0g
Sorbitan monooleate complex ..................................................1.0g
MgSO4 ·7H2O..................................................................0.4g
DL- Tryptophan................................................................0.2g
L- Cystine ...................................................................0.2g
FeSO4 .......................................................................0.02g
MgSO4 ·7H2O.................................................................0.02g
NaCl.........................................................................0.02g
Adenine sulfate.............................................................0.018g
Guanine·HCl................................................................0.012g
Uracil ......................................................................0.01g
Xanthine.....................................................................0.01g
Pyridoxal....................................................................4.0mg
Pyridoxine...................................................................4.0mg
Calcium pentothenate ........................................................2.0mg
Niacin.......................................................................2.0mg
Riboflavin ..................................................................2.0mg
Thiamine·HCl ...............................................................2.0mg
Folic acid...................................................................1.0mg
Biotin .....................................................................1.0μg
Lactobacillus leichmannii suspension.........................................1.0mL
pH 6.2 ± 0.1 at 25°C
Preparation of Medium: Add components, except Lactobacillus
leichmannii suspension, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Inoculate me-
dium with 1.0mL of Lactobacillus leichmannii suspension. Mix thor-
oughly. Pour into sterile 150mm Petri dishes in 50.0mL volumes.
Allow agar surface to dry before using.
Use: For the microbiological assay of vitamin B 12 by the cup plate or
disk method using Lactobacillus leichmannii as the test microorgan-
ism.