Crystal Violet Tetrazolium HiVeg Agar Base

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Crystal Violet Tetrazolium HiVeg Agar Base

培养基配方
Composition per liter:
Agar.........................................................................15.0g
Plant hydrolysate ............................................................5.0g
Yeast extract.................................................................2.5g
Glucose.......................................................................1.0g
Crystal Violet...............................................................1.0mg
2,3,5-Triphenyltetrazolium chloride solution................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without triphenyltetrazolium chloride solution,
is available as a premixed powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
2,3,5-Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium chloride...........................................0.1g
Preparation of 2,3,5-Triphenyltetrazolium Chloride Solu-
tion: Add 2,3,5-triphenyltetrazolium chloride to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except 2,3,5-triphe-
nyltetrazolium chloride solution, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 10 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL 2,3,5-triphenyltetrazolium chloride solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the detection of Gram-negative psychrotrophic bacteria caus-
ing food spoilage.