Crystal Violet Azide Esculin Agar

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Crystal Violet Azide Esculin Agar

培养基配方
Composition per liter:
Agar.........................................................................15.0g
Glucose.......................................................................5.0g
NaCl..........................................................................5.0g
Proteose peptone..............................................................5.0g
Pancreatic digest of casein...................................................5.0g
Meat extract..................................................................3.0g
Esculin.......................................................................1.0g
NaN3 .........................................................................1.0g
Crystal Violet................................................................0.1g
Bovine blood, citrated.....................................................100.0mL
pH 7.5 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components, except citrated bovine
blood, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile, cit-
rated bovine blood. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation of Erysipelothrix rhusiopathiae.