Crystal Violet Agar

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Crystal Violet Agar

培养基配方
Composition per liter:
Agar ..............................................................................15.0g
Lactose............................................................................10.0g
Proteose peptone....................................................................5.0g
Beef extract........................................................................3.0g
Crystal Violet.....................................................................3.3mg
pH 6.8 ± 0.1 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation of pathogenic staphylococci from non-
pathogenic staphylococci. Hemolytic and coagulating strains of Staph-
ylococcus aureus appear as purple or yellow colonies. Nonhemolytic
and noncoagulating strains of Staphylococcus species appear as white
colonies.