Crystal Violet Esculin Agar

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Crystal Violet Esculin Agar

培养基配方
Composition per liter:
Agar..........................................................................15.0g
Glucose........................................................................5.0g
NaCl...........................................................................5.0g
Proteose peptone...............................................................5.0g
Pancreatic digest of casein....................................................5.0g
Meat extract...................................................................3.0g
Esculin........................................................................1.0g
Crystal Violet................................................................2.0mg
Blood, citrated..............................................................100.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except citrated blood,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add citrated blood.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of Erysipelothrix rhusiopathiae.