CRYS Medium

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CRYS Medium

培养基配方
Composition per liter:
Stock extract ..........................................................500.0mL
2× PP medium...........................................................500.0mL
Stock Extract:
Composition per 500.0mL:
Cerophyll ................................................................5.0g
Brown rice ...............................................................5.0g
Yeast extract.............................................................5.0g
Dried seaweed.............................................................5.0g
Preparation of Stock Extract: Add components to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly. Gently heat
and bring to boiling. Continue boiling for 5 min. Filter three times
through Whatman #1 filter paper while still hot. Cool to room temper-
ature. Adjust pH to 7.2. Bring volume to 500.0mL with distilled/deion-
ized water. Autoclave for 15 min at 15 psi pressure–121°C.
2 × PP Medium:
Composition per 500.0mL:
Proteose peptone.........................................................10.0g
Pancreatic digest of peptone.............................................10.0g
Ribonucleic acid from Torula yeast.......................................1.0g
Asolectin................................................................0.2g
Artificial seawater...................................................167.0mL
Vitamin solution........................................................2.0mL
Artificial Seawater:
Composition per 167.0mL:
Aqua-Marin sea salts ..................................................6.95g
Source:  Aqua-Marin sea salts are available from Aquatrol, Inc., Ana-
heim, CA.
Preparation of Artificial Seawater: Add Aqua-Marin sea salts to
distilled/deionized water and bring volume to 167.0mL Mix thorough-
ly. Filter sterilize.
Vitamin Solution:
Composition per 100.0mL:
Thiamine·HCl ........................................................150.0mg
Calcium  D -(+)-pantothenate..........................................100.0mg
Folic acid.............................................................50.0mg
Nicotinamide...........................................................50.0mg
Pyridoxal·HCl.........................................................50.0mg
Riboflavin ............................................................50.0mg
DL -6-Thioctic acid.....................................................1.0mg
Biotin solution........................................................10.0mL
Biotin Solution:
Composition per 10.0mL:
Biotin .................................................................0.01mg
Preparation of Biotin Solution: Add biotin to 10.0mL of absolute
ethanol. Mix thoroughly.
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize. For long-term storage, preserve under nitrogen at −20°C.
Preparation of 2 × PP Medium: Add asolectin to 200.0mL of dis-
tilled/deionized water. Gently heat to 80°C. Mix thoroughly. Add other
components, except artificial seawater and vitamin solution, to dis-
tilled/deionized water and bring volume to 331.0mL. Mix thoroughly.
Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically add 167.0mL of sterile artificial seawater and 2.0mL of
sterile vitamin solution. Mix thoroughly.
Preparation of Medium: Aseptically combine 500.0mL of sterile
stock extract with 500.0mL of sterile 2× PP medium. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Pseudocohnilembus marinus.