Creatinine Agar

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Creatinine Agar

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Na2HPO4 ·12H2O...............................................................9.0g
NaCl..........................................................................5.0g
KH2PO4 .......................................................................1.5g
Creatinine....................................................................1.0g
Meat extract .................................................................1.0g
Yeast extract.................................................................1.0g
MgSO4 ·7H2O..................................................................0.2g
MnCl2 ·4H2O ...............................................................20.0mg
CaCl2 .......................................................................1.2mg
Glucose solution ..........................................................100.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose .....................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize. Warm to 50°C.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of
sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas species
and other bacteria that can utilize creatinine.