CREA (Creatine Agar)

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CREA
(Creatine Agar)

培养基配方
Composition per liter:
Sucrose......................................................................30.0g
Agar.........................................................................15.0g
Creatine·H2O.................................................................3.0g
K3P04 ·7H2O..................................................................1.6g
Bromcresol Purple...........................................................50.0mg
Minerals solution...........................................................10.0mL
Trace minerals solution......................................................1.0mL
Minerals Solution:
Composition per 100.0mL:
KCl...........................................................................5.0g
MgSO4 ·7H2O..................................................................5.0g
FeSO4 ·7H2O..................................................................0.1g
Preparation of Minerals Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Minerals Solution:
Composition per 100.0mL:
ZnS04 ·7H2O...................................................................1.0g
CuSO4 ·5H2O...................................................................0.5g
Preparation of Trace Minerals Solution:  Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Penicillium species.