Creatinine Medium

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Creatinine Medium

培养基配方
Composition per liter:
Creatinine....................................................................5.0g
Agar .........................................................................2.0g
Fumaric acid .................................................................2.0g
K2HPO4 .......................................................................2.0g
Yeast extract.................................................................1.0g
Salt solution ...............................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Salt Solution:
Composition per liter:
MgSO4 .........................................................................12.2g
FeSO4 ·7H2O....................................................................2.8g
MnSO4 ·H2O.....................................................................1.7g
CaCl2 ·2H2O ..................................................................0.76g
NaCl............................................................................0.6g
Na2MoO4 ·2H2O..................................................................0.1g
ZnSO4 ·7H2O...................................................................0.06g
HCl (0.1N solution) ............................................................1.0L
Preparation of Salt Solution: Dissolve salts in 1.0L of 0.1N HCl
solution. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8 with
NaOH or KOH. Gently heat until boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Pseudomonas species.