Clostridium Medium (ATCC Medium 163)

[所属分类:培养基配方] [发布时间:2020-5-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Medium
(ATCC Medium 163)

培养基配方
Composition per liter:
Agar .....................................................................20.0g
Sodium glutamate..........................................................17.0g
Yeast extract..............................................................6.0g
Sodium thioglycolate.......................................................0.5g
Phosphate buffer (1.0M, pH 7.4)..........................................40.0mL
MgSO4 (2.0M solution) ....................................................0.5mL
FeSO4 (0.2M solution)....................................................0.2mL
CaCl2 (1.0M solution).....................................................0.1mL
CoCl2 (0.1M solution).....................................................0.1mL
MnCl2 (0.1M solution).....................................................0.1mL
Na2MoO4 (0.1M solution) ..................................................0.1mL
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of a variety of Clostridium
species.