Clostridium Medium (ATCC Medium 591)

[所属分类:培养基配方] [发布时间:2020-5-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Medium
(ATCC Medium 591)

培养基配方
Composition per liter:
Solution 1.............................................................600.0mL
Solution 2.............................................................400.0mL
pH 8.0 ± 0.2 at 25°C
Solution 1:
Composition per 600.0mL:
Peptone...................................................................5.0g
Preparation of Solution 1: Add peptone to distilled/deionized wa-
ter and bring volume to 600.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution 2:
Composition per 400.0mL:
NaHCO3 ..................................................................20.0g
Fructose.................................................................10.0g
K2HPO4 ..................................................................10.0g
Sodium thioglycolate.....................................................0.75g
Vitamin solution........................................................14.0mL
Trace elements solution ................................................10.0mL
Preparation of Solution 2: Add components, except sodium thio-
glycolate, to distilled/deionized water and bring volume to 400.0mL.
Mix thoroughly. Gas with 100% CO2 . Add sodium thioglycolate. Ad-
just pH to 8.0. Filter sterilize.
Vitamin Solution:
Composition per 100.0mL:
Thiamine.................................................................0.1g
Nicotinic acid..........................................................0.05g
Pyridoxine..............................................................0.05g
Pantothenic acid.......................................................0.025g
p-Aminobenzoic acid.....................................................5.0mg
Vitamin B 12 ...........................................................2.0mg
Biotin .................................................................1.0mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
EDTA.....................................................................0.5g
FeSO4 ·7H2O.............................................................0.2g
H3BO3 ..................................................................0.03g
CoCl2 ·6H2O ...........................................................0.02g
ZnSO4 ·7H2O............................................................0.01g
MnCl2 ·4H2O............................................................3.0mg
Na2MoO4 ·2H2O .........................................................3.0mg
NiCl2 ·6H2O............................................................2.0mg
CuCl2 ·2H2O ...........................................................1.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Aseptically combine 600.0mL of sterile
solution 1 and 400.0mL of sterile solution 2. Distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of a variety of Clostridium
species.