Clostridium Medium (ATCC Medium 43)

[所属分类:培养基配方] [发布时间:2020-5-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Medium
(ATCC Medium 43)

培养基配方
Composition per liter:
Agar .....................................................................15.0g
Yeast extract..............................................................5.0g
L- Arginine·HCl...........................................................2.0g
L- Lysine·HCl.............................................................2.0g
NH4Cl .....................................................................2.0g
Sodium formate.............................................................2.0g
K2HPO4 ...................................................................1.75g
MgSO4 ·7H2O...............................................................0.2g
CaCl2 ·2H2O..............................................................0.01g
FeSO4 ·7H2O..............................................................0.01g
Methylene Blue............................................................2.0mg
Na2S·9H2O solution......................................................30.0mL
Na2S·9H2O Solution:
Composition per 100.0mL:
Na2S·9H2O.................................................................1.0g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 100.0mL. Autoclave for 15
min at 15 psi pressure–121°C. Use freshly prepared solution.
Preparation of Medium: Add components, except Na2S·9H2O so-
lution, to tap water and bring volume to 1.0L. Mix thoroughly. Gently
heat to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Distribute anaerobically into sterile tubes. Aseptically
add 0.15mL of sterile Na2S·9H2O solution to each 5.0mL of the medi-
um. Cap with rubber stoppers.
Use:  For the cultivation and maintenance of a variety of Clostridium
species.