Clostridium Medium

[所属分类:培养基配方] [发布时间:2020-5-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Medium

培养基配方
Composition per liter:
Uric acid................................................................2.0g
Yeast extract............................................................1.2g
MgSO4 ·7H2O............................................................0.05g
CaCl2 ·2H2O ...........................................................5.0mg
FeSO4 ·7H2O............................................................2.0mg
Resazurin...............................................................1.0mg
KOH (10N solution) .....................................................3.0mL
K2HPO4 ·3H2O (70% solution) ...........................................1.5mL
Mercaptoacetic acid.....................................................1.5mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add KOH solution and K2HPO4 ·3H2O
solution to distilled/deionized water and bring volume to 500.0mL.
Gently heat and bring to boiling. Mix thoroughly. Add uric acid slowly.
Cool to 45°–50°C. Add remaining components. Add mercaptoacetic
acid immediately prior to sterilization. Bring volume to 1.0L with dis-
tilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Adjust pH to 7.2 with sterile 60% K2CO3 solution.
Use: For the isolation and cultivation of purine-fermenting Clostrid-
ium species.